| QuickPick™ SML genomic DNA 51002 • genomic DNA purification kit, 8 preps 51012 • genomic DNA purification kit, 24 preps 51022 • genomic DNA purification kit, 96 preps |
The QuickPick™ SML gDNA purification kits are intended for universal genomic DNA purification from various sample materials. DNA can be purified for example from human or animal whole blood, buffy coat, leukocytes, dried blood spots, tissue, cultured cells, bacteria, hair, buccal swabs, urine, saliva, milk and seminal fluid. The reagents are suitable for use with whole blood which has been treated with commonly used anticoagulants like EDTA, heparin or citrate. The technique provides a fast and simple means of DNA purification and does not require any organic solvents and eliminates the need for repeated centrifugation, vacuum filtration or column separation. The size of DNA purified using the QuickPick™ SML gDNA purification kit is typically at least 30 kbp. |
| QuickPick™ SML Plant DNA 53002 • Plant DNA purification kit, 8 preps 53012 • Plant DNA purification kit, 24 preps 53022 • Plant DNA purification kit, 96 preps |
The QuickPick™ SML Plant DNA purification kits provide fast and simple means of purifying genomic DNA from a variety of plants or their organelles. The technique does not require any organic solvents and eliminates the need for repeated centrifugation, vacuum filtration or column separation. The size of DNA purified using the QuickPick™ SML Plant DNA purification kits is typically at least 30 kbp. DNA fragments of this length denature completely during thermal cycling and can be used for downstream applications such as PCR amplifications, restriction enzyme digestions and sequencing. |
| QuickPick™ SML total RNA 42002 • total RNA purification kit, 8 preps 42012 • total RNA purification kit, 24 preps 42022 • total RNA purification kit, 96 preps |
The QuickPick™ SML total RNA purification kits provide a fast and simple means of purifying total RNA from whole blood, tissues or plant tissues. The method does not require any organic solvents and eliminates the need for repeated centrifugations, vacuum filtration or column separation. The purified total RNA can be used for downstream applications such as RT-PCR. |
| QuickPick™ SML mRNA 41002 • mRNA purification kit, 8 preps 41012 • mRNA purification kit, 24 preps 41022 • mRNA purification kit, 96 preps |
The QuickPick™ SML mRNA purification kits provide a fast and simple means of purifying poly-A(+) mRNA from cultured cells, total RNA and animal or plant tissues. The method does not require any organic solvents and eliminates the need for repeated centrifugations, vacuum filtration or column separation. The purified mRNA can be used for down-stream applications such as RT-PCR, real-time PCR, cDNA libraries and microarray analyses. |
| QuickPick™ DNA Fragment 71001D • DNA Fragment DEMO kit, 24 preps 71011 • DNA Fragment kit, 3 x 96 preps |
The QuickPick™ DNA Fragment kit provides a fast and simple means of isolating DNA fragments from gel or from liquid solutions. The kit and Bio-Nobile's PickO™ gel excisers are suitable for use with standard and low-melting agarose gels in TAE or TBE buffer. The technique does not require organic solvents and eliminates the need for repeated centrifugation, vacuum filtration or column separation. Purified DNA fragments can be used for downstream applications such as automated fluorescent DNA sequencing, manual DNA sequencing, PCR, in vitro transcription/translation, restriction mapping, restriction enzyme digestion, cloning and labelling. |
| QuickPick™ IMAC 62301 • metal affinity kit for proteins, 8 preps 62311 • metal affinity kit for proteins, 48 preps |
The QuickPick™ IMAC kits provide a fast and simple means for recombinant histidine-tagged (His-tag) protein purification based on metal affinity using Ni2+. Immobilized metal ion affinity chromatography (IMAC) was originally introduced as a method for group separation, but today it represents one of the most important tools for the single-step purification of proteins based on polyhistidine tags. The principle of the mechanism is based on the interaction between a metal ion coordinated to a covalently bound chelating ligand and a His-tag protein. |
| QuickPick™ CM 62201 • weak cation exchange kit for proteins, 8 preps 62211 • weak cation exchange kit for proteins, 48 preps QuickPick™ DEAE 62101 • weak anion exchange kit for proteins, 8 preps 62111 • weak anion exchange kit for proteins, 48 preps |
The QuickPick™ CM and DEAE kits are based on magnetic cellulose particles coated with carboxymethyl (CM) groups or diethylaminoethyl (DEAE) groups. Different types of proteins will bind to the coated magnetic particles with affinities that depend on both the conditions used and the types and number of individual charged groups. Typically a mixture of numerous proteins, for example cell lysate, is used as a sample with ion exchangers. After unbound molecules are washed away, the composition of the buffer is changed by altering the pH and salt concentration to release the molecule(s) of interest from the particles. |
