Bio-Nobile genomic DNA purification solutions
The QuickPick gDNA kit is universal and suitable for purifying genomic DNA from a wide variety of starting materials such as human and animal blood, buffy coat, leukocytes, hair, saliva, cultured cells, tissue, swap, buccal cells, urine and bacteria. DNA purified using QuickPick gDNA reagents show a recovery of at least 30 kbp which is suitable for all typical downstream applications, including: PCR, sequencing, RE digestion and cloning.
|
Sample |
Amount |
Yield |
Amount |
Yield |
Amount |
Yield |
|
Whole Blood |
50 µl |
Up to 1.5 µg |
100 µl |
Up to 3 µg |
200 µl |
Up to 6 µg |
|
Buffy Coat |
50 µl |
Up to 3 µg |
100 µl |
Up to 6 µg |
|
|
|
Leukocytes |
0.5 million |
Up to 4 µg |
1 million |
Up to 8 µg |
|
|
|
Cultured Cells |
1 million |
Up to 2.5 µg |
2 million |
Up to 5 µg |
4 million |
Up to 10 µg |
|
Tissue (liver) |
2.5 mg |
Up to 8 µg |
5 mg |
Up to 16 µg |
10 mg |
Up to 32 µg |
|
Hair |
2-4 strands |
Up to 0.5 µg |
6-8 strands |
Up to 1 µg |
|
|
Typical purity = 1.7
Size of purified DNA = 30 kbp
Yields will depend on species, growth conditions, etc.
Leukocytes were suspended in 50-200 µl of PBS depending on sample amount
* Ratio of absorbance at 260/280 nm is corrected with absorbance at 320 nm
Increasing the sample amount will lead to increasing yield.
Typical performance of the QuickPick™ Plant DNA kit:
